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Furthermore, broilers supplemented with 300 mg/kg APS improved the intestinal mucosal immune barrier function of broilers by enhancing mRNA expression of Occludin, Claudin-1, ZO-1, and MUC2 in small intestine, and improved growth by enhancing concentration of immunoglobulins (Ig) A, IgM and IgG, and lowing concentrations of TNF-, IL-1, IL-6, and diamine oxidase (DAO) in serum (74)

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Furthermore, for specific compounds, additional key mechanisms such as Fas-mediated signal transduction, loss of cellular redox homeostasis, ATP depletion, changes in ion channels, and activation of kinases and caspases, among others, have been highlighted

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However, these methods face practical hurdles, as RNA is highly susceptible to degradation during sampling and processing, especially in low-biomass environments such as those treated with biocides, making it difficult to obtain RNA of sufficient quantity and quality 24