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Based on preliminary results (data not shown) the chemical shift of the pyridinium ring-bound hydrogens vary greatly with the protonation of the basic moieties, therefore the electron density around the pyridinium ring is certainly perturbed

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[DOI] [PMC free article] [PubMed] [Google Scholar] van de Steeg E, Wagenaar E, van der Kruijssen CM, Burggraaff JE, de Waart DR, Elferink RP, Kenworthy KE, and Schinkel AH (2010) Organic anion transporting polypeptide 1a/1b-knockout mice provide insights into hepatic handling of bilirubin, bile acids, and drugs