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For the second step of trypanothione synthesis glutathionylspermidine is bound in a way that preferentially allows N1-glutathionylation of N8-glutathionylspermidine, classifying N8-glutathionylspermidine as the favoured substrate

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It is commonly prescribed for individuals with pernicious anemia, certain gastrointestinal disorders, or dietary deficiencies that impair B12 absorption

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For complete details on the use and execution of this protocol, please refer to Hess etal.1.