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Protein bands were visualized with an ECL detection kit, and the intensity of protein bands was quantitated by Image Lab 6.1 software (Bio-Rad Laboratories, Hercules, CA)

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The overfeeding diets have greater effects on the amino acid metabolism and autophagy activation of zebrafish [47, 230], and mRNA expressions of genes associated with lipogenesis and glucose metabolism, such as lipin1 , srebf1 , and irs2a , are decreased [47, 230]

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