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For scRNA-seq library preparation, cells were loaded onto the 10 Genomics Chromium instrument and 10 Genomics Chromium Single-Cell 3kit V3 reagents were used according to the manufacturers protocols

28 However, oral therapy does not improve serum methylmalonic acid levels as well as intramuscular therapy, although the clinical relevance is unclear

[DOI] [PubMed] [Google Scholar] 126.Baier P.C., Weinhold S.L., Huth V., Gottwald B., Ferstl R., Hinze-Selch D

Each reaction mixture was prepared with SYBR Green-containing master mix (Takara Bio Inc.), primers specific for the target genes (Table 1), and a cDNA template