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Immunoblot To measure Akt phosphorylation, confluent bFLS were treated with bTNF- for 5 min at 37 C under 5% CO 2

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Briefly, subclasses of KO metabolic proteins were organized based on location in the soluble or insoluble fraction and connected to protein nodes with red or blue edges based on increasing or decreasing abundance, respectively

Your provider can order appropriate testing and interpret results in context of your health history and goals

However, there is a risk that additional surgery may be necessary in the future