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Table 2) were tested by using a standard curve of pooled cDNA and nuclease-free water ranging from 1:2 to 1:256 prior to use

Deficiency in vitamin B12 can lead to the accumulation of methylmalonyl-CoA, which is subsequently converted to methylmalonic acid (MMA) (70, 73)

FODMAP Diet and the Microbiome A common concern is that restricting FODMAPs may reduce beneficial prebiotics like inulin and fructooligosaccharides (FOS)

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The methods were performed in accordance with the approved guidelines from Huazhong Agricultural University, and scientific, ethical and legal principles of the Hubei Regulations for the Administration of Affairs Concerning Experimental Animals