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Biotinylated goat anti-mouse IgG or goat anti-rabbit IgG (Vector Labs, BA-9200 1:200 and BA-1000 1:200, respectively) secondary antibodies were added in blocking buffer and incubated for 1 h at room temperature

Cofactor formation was much slower than the rates reported for other amino acid cofactor-containing enzymes and, in fact, took hundreds of catalytic turnover cycles to occur

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doi: 10.1080/09712119.2015.1091339 109

10.1016/j.jaad.2012.12.781 123 InoueY.IsobeM.HayashiH

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