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Thawing Cryopreserved PBMCs The vials of cryopreserved PBMCs in FBS 10% DMSO O were transferred from -80C storage on dry ice to a warm water bath heated to 37C

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Eur J Med Chem 73:153166 Domokos-Szabolcsy E, Marton L, Sztrik A, Babka B, Prokisch J, Fari M (2012) Accumulation of red elemental selenium nanoparticles and their biological effects in Nicotinia tabacum

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Briefly, the frozen cortical samples were homogenized in SDT-lysis buffer (100 mM TrisHCl (pH 7.6) containing 0.1 M DTT) using an ultrasonic homogenizer, and the homogenates were heated at 95 C for 3 min