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HEK-293T cells were grown in high glucose (4.5 g/L glucose) Dulbecco's modified Eagle's medium (DMEM, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS, Natocor), penicillin/streptomycin (100 units/mL and 100 g/mL, respectively, Thermo Fisher Scientific) and 110 mg/L of sodium pyruvate (Thermo Fisher Scientific) in a 37 C humidified incubator containing 5% CO 2
The exact minimum effective concentration that recapitulates our observed suppressive ILC2 phenotype in vivo , remains to be determined, and will likely rely on a complex grouping of factors that take into account systems with endogenous melatonin rhythms (such that were absent in our studies both in vitro and in vivo with BALBc mice)
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[DOI] [PMC free article] [PubMed] [Google Scholar] El-Shabrawi H, Kumar B, Kaul T, Reddy MK, Singla-Pareek SL, Sopory SK
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