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After resuspended with CD31 and 9 times the volume of Buffer, shaken for 15 min, passed through the MACS column, Buffer washed twice, the column was taken off and 500 LBuffer was quickly added to blow the cells into a clean 1.5 mL EP tube, centrifuged and supernatant removed, this cell is the vascular endothelial cell

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[DOI] [PMC free article] [PubMed] [Google Scholar] 114.Rodrigues L.A.G., Hernandez-Dias S

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Microinfusion's hollow microneedles bypass this barrier at a fixed 0.5mm depth