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Cells were lysed with 500 L of RIPA lysis buffer containing 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 25 mM Tris-HCl, supplemented with protease and phosphatase inhibitors at a 1:100 dilution

Anal Chim Acta 486 : 5161 Li KM, Rivory LP, Clarke SJ (2005) Rapid quantitation of plasma 2-deoxyuridine by high-performance liquid chromatography/atmospheric pressure chemical ionization mass spectrometry and its application to pharmacodynamic studies in cancer patients

Over the past few decades, bone regeneration research has discovered that certain peptides might be able to play a role in speeding up the bone healing response [2]

References Daniels, E.W., Cole, D., Jacobs, B

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Sci Rep 14, 29621.Google Scholar Matsuda, Y, Ozawa, N, Shinozaki, T et al