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For gene expression analysis, TaqMan Gene Expression Assays (Applied Biosystems, Thermo Fisher Scientific, Massachusetts, USA) were used (Table 1, additional material)

10.1038/nrdp.2016.69 56 EastellR.WalshJ

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Despite the importance of TJs in cellcell adhesion, it is the more basal desmosome structures that are primarily responsible for the mechanical strength of the intestinal epithelium by acting as spot-welds that clutch cells together (Roxas and Viswanathan 2018)
