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Death of GSCs was apparently due to the iron dependent form of programmed cell death characterized by the accumulation of lipid peroxides called ferroptosis

Lancet (2017) 390:155062

doi: 10.1089/ars.2022.0214

Briefly, cotton stoppered shake-flasks were used with a working mass of 50 g containing water insoluble solids (WIS) content of 5 or 10% (w/w) steam-pretreated spruce (SEKAB E-Technology rnskldsvik, Sweden)