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Glyceraldehyde-3-Phosphate Dehydrogenase The reaction velocity was measured as an increase in absorption at 340 nm resulting from the reduction of NAD in a cuvette, containing 10 mM sodium phosphate buffer (with the addition of 20 mM sodium arsenate), pH 8.5, 0.25 mM NAD + , 3 mM DTT and 0.25 M D-glyceraldehyde-3-phosphate

Vitamin B12, which is present in a soluble state when taken in liquid form, can be easily absorbed swiftly without the need for any further processing

Cell pellets were resuspended in 0.4 ml methanol (Nacalai), then 0.275 ml of MilliQ containing 125 ng/ml BIS-TRIS (Sigma-Aldrich) was added to the methanol

X-ray structural analysis indicates that, in the reduced form, active site Cys473 and backdoor Cys426 are separated by 4.6 (Fig

It exists primarily in its active, reduced form (GSH), which is pivotal for cellular defense and detoxification

Psychiatry 15, 272285