glutathione s transferase fusion protein Transglutaminase 2 crosslinks the S-transferase tag, impeding protein–protein interactions of the fused Frontiers | Glutathione S-Transferase P1

The membrane was exposed to a Kodak X-ray film, and densitometry of the spots was performed using the Fluor-S MultiImage system (Bio-Rad)

Experiments were analyzed in a blinded manner and all behavioural tests were independently replicated at least once

Pharmacol Rev 54:101127 Talebi S, Bolhassani A, Azad TM, Arashkia A, Modaresi MH (2017) Immuno-stimulating peptide derived from HMGB1 is more effective than the N-terminal domain of Gp96 as an endogenous adjuvant for improvement of protein vaccines

The plate was then fixed with 4% paraformaldehyde for 10 minutes at room temperature

At the centre of the molten pool a crater can be observed where the focal point of the laser was targeted

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