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The well content was quickly detached using a cell scraper and collected in microcentrifuge tube (Sarstedt), directly snap-frozen in liquid nitrogen, and stored at 80 C until further use
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In the presence of uncouplers (small molecules or proteins), protons return to the mitochondrial matrix bypassing ATP synthase
68 , 82 It is unlikely, however, that rising blood levels of the cytokines TNF, IL-6, and IL-8 initiate the JHR because the chill and temperature rise start as early as 30 minutes to 1 hour after antibiotic treatment, 28 , 68 , 73 when blood levels of these cytokines, already elevated before antibiotic treatment, stay about the same for 30 minutes to 1 hour after treatment
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22 Liberini, C
