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Protein extraction and immunoblotting For immunoblot analyses, total protein was extracted from 4-day-old seedlings as previously described 55

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J Clin Invest 120(9):33773388

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[206] [207] [208] [209] [210] On February 28, HHS's top spokesperson, Thomas Corry, abruptly resigned, two weeks after being sworn in as the assistant secretary of public affairs

Nearly 80% TAT-CcSOD1 existed in form of soluble protein at 12 C that was determined as the optimal temperature for TAT-CcSOD1 expression in subsequent experiments