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Each animal was inoculated by subcutaneous injection of 2 10 6 HT-29 cells mixed with 1 10 6 freshly isolated human PBMCs, cells were mixed 1:1 v/v with LDEV-free extracellular matrix protein Geltrex (Gibco, # A1413201) and inoculated in the right flank under anaesthesia

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Yang SH, Wu CC, Shih TT, Sun YH, Lin FH: In vitro study on interaction between human nucleus pulposus cells and mesenchymal stem cells through paracrine stimulation

In that last case, a removable-radial-arm maze structure (6 or 8 arms) and associated platform can be provided upon request