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Quantitative real-time PCR (qRT-PCR) analysis was performed on a CFX 96 BioRad cycler in triplicate with SensiFAST SYBR Green Kit (Catalog Number BIO-98005, USA), according to the manufacturers instructions

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(Diagram not to scale) / CC BY 3.0 Blood Cholesterol Recommendations For healthy total blood cholesterol, the desired range you would want to maintain is under 200 mg/dL

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