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Evidence suggests that in the early phase of viral infection perturbation of host cell membrane decreases of intra cellular pH and GSH efflux which allow to commence the viral cycle in the host cell
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that is, blood levels increase in proportion to the dose and decrease in proportion to the concentration
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For Kd estimation, a slow-exchange binding model was utilized, allowing for the evaluation of residue-specific isotherm curves by tracking changes in peak intensity during the titration
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