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When two groups were compared, a two-tailed unpaired Students t -test was applied

Moreover, peptides can be degraded in the oral cavity due to the variable pH of the saliva, unless supported by advanced formulation such as mucoadhesive systems, permeation enhancers, or nano-enabled delivery platforms like liposomes, niosomes and polymeric nanoparticles [66]

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Key limitation: absence of dosing studies, pharmacokinetic data, or controlled human sleep outcomes

The saline composition and bacteriostatic preservative system work together to support the stability of sensitive compounds during storage and in vitro experimentation