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coli and fast frozen under high pressure using a Balt-tec HM 010 freezing device, placed into 2% osmium tetroxide, 0.1% UAc, 2% dH2O in acetone at -90C for 96 hrs using a Boeckler freeze substitution unit, slowly warmed (5C per hr) to -30C, held 16 hrs, then slowly warmed (5C per hr) to 0C, then rinsed in cold acetone at 0C, and again rinsed several more times at room temp before infiltration into plastic resin over 3 days (HardPlus Embed812)

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