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Description
[PubMed: 18331606] 52

Can use continuously or cycle 12 weeks on, 4 weeks off
Using regular sterile water instead of bacteriostatic water provides no antimicrobial protection, allowing bacterial growth that contaminates the entire vial

The adult rat is the species of choice due to its well-documented physiological responses, established baseline toxicology data, and extensive use in regulatory testing frameworks
Several developments on the horizon could shape how this combination is studied and potentially applied
Boyd SD, Ullrich MS, Skopp A, Winkler DD