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Theyre a popular choice for a reason, but they arent the only optionand for many people, they arent the most effective one
So, for him to now go into a fight against an active contender with what appears to be a huge disadvantage, just seems like a waste of everybodys time

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Use of the fluorescent dye 10-N-nonyl acridine orange in quantitative and location assays of cardiolipin: a study on different experimental models

J Biol Chem , 293: 1310013111, 2018

In the second step, barcodes from the Nextera XT kit were attached