glutathion sepharose glutathione resin pC6-2/caspase-6 system to purify glutathione-S-transferase-free recombinant fusion proteins expressed in Escherichia coli via a 10 Glutathione S-transferase assay. Denaturing SDS-PAGE

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The bottom-up approach is less favored because of the challenges of eliminating solvent residues

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