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Bruder was attacked while swimming 130 yards (120 m) from shore

Cell culture and transfection WT HepG2 or stably transfected cells (pSuperior.puro-GSTM2) were cultured in DMEM (HyClone, Logan, UT, USA) containing 10% foetal bovine serum (Clark Bioscience, Richmond, VA, USA), 100 U/mL penicillin, and 100 g/mL streptomycin in dishes at 37 C and transfected with Lipo6000 Transfection Reagent (#C0528, Beyotime)

Note: Liposomal delivery is designed to support absorption at the cellular level by mimicking the body's natural cell structures, allowing the nutrient to pass through the digestive tract intact

Riederer et al., 2021

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Possible interaction with HPA-axis hormones