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Equal amounts of total protein (30 g per sample) were resolved by 1012% SDS-PAGE and subsequently transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore or Invitrogen, Shanghai, China)

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Croatian researcher Predrag Sikiric and colleagues at the University of Zagreb have published the bulk of the animal research over roughly three decades, with studies covering tendon healing, gastric ulceration, colitis, brain injury, and systemic organ protection in rodent models

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