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Transcriptional responses to Cys depletion To gain insights into the molecular responses triggered by cysteine deprivation, we conducted bulk RNA-sequencing (RNA-seq) analysis of liver (which exhibits the highest expression of Cse ), muscle (the most abundant tissue) and adipose tissue (the tissue most impacted by cysteine loss)

For these mutations we can visualize more stringent constraints

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To acquire energy, FA activation is a must, where FAs are converted into acyl-CoA by the acyl-CoA synthetase on the outer mitochondrial membrane, followed by entering mitochondria with the assistance of carnitine palmitoyltransferase 1 (CPT1) on the outer mitochondrial membrane together with carnitine-acylcarnitine translocase (CACT) and carnitine palmitoyltransferase 2 (CPT2) on the inner membrane [112]