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The improvement was evident in several metabolic markers, including reduced levels of propionylcarnitine, ammonia, and methylcitrate, as well as decreased ratios of propionylcarnitine/acetylcarnitine (C3/C2) and methylcitrate/citrate

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The bladder and prostate were gently moved aside to expose the cavernous nerve, which was electrically stimulated using a 25G bipolar platinum electrode (Biopac Systems Inc., Santa Barbara, CA, USA) positioned under the nerve, and a 25G needle was used to introduce a pressure transducer into the exposed penile crus (Biopac Systems Inc., Santa Barbara, CA, USA)