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doi: 10.1016/S1734-1140(11)70600-4

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Depending on the experiment, a culture of 100 mL or of 2 L Luria-Bertani (LB) medium supplemented with 100 g/mL ampicillin (LB/Amp) was inoculated with 1/100 volume of an overnight culture and grown at 30 C to OD 600 0.4

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