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After preparing pH of the medium (~6.8) to 6.9 by adding ammonia solution (28%) (Nacalai Tesque), the culture was started by adding the above-mentioned inoculum into the medium
Briefly, 2 l of 1 g l 1 CpG-A or CpG-B or vehicle were mixed with 98 l of sterile PBS
Overall iron absorption is low
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Overall the regulation of GSH maintenance, binding of GSH to proteins, its oxidation and release is complicated
