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Eijk et al
Post-fixation processing was performed in 1% osmium tetroxide for 2 h
The brains of the mice were removed, postfixed with the same fixative for 24 h at 4C, and then cryoprotected using a graded series of buffered sucrose solutions (20% and 30%)
Secondly, there are issues with the comprehensiveness of the study designs
Of note, one ND individual was later identified to have maturity-onset diabetes of the young
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