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To estimate the concentration of serum proteins, Megabrix portable manual refractometer (IONLab Barigui, Brazil) was used

PMID: 11134975 Chinta SJ, Kumar MJ, Hsu M, Rajagopalan S, Kaur D, Rane A, Nicholls DG, Choi J, Andersen JK

By monitoring changes in absorbance upon ligand binding, we can help determine the binding constants, identify conformational changes in the protein, and assess the nature of the interaction

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This means semaglutide requires much smaller syringe volumes at comparable concentrations

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