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The density of small tight junction pores varies among cell types and is increased by expression of claudin-2

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The exact means of defining this population are out of scope for this document

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Put plainly, the off period is not there because the compound stops working it is there because no one can point to human data showing that running it indefinitely is safe, and a scheduled break is the honest way to respect that gap

While melt shift analysis helps identify proteins with biophysical changes, TPCA is a method that more specifically monitors binary protein interactions and protein complex dynamics (75)