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10.1152/ajprenal.00617.2018 Am
doi:10.1136/bmj.324.7352.1512 Song C, He J, Chen J, et al
Preparation of protein lysates The cells collected from 6-well plates were lysed in 0.4 ml lysis buffer (20 mM Tris-HCl, pH 7.5, 150 mM NaCl, and 2% SDS) followed by sonication (10 cycles of 10 s pulses with 10 s rest intervals) using a Fisher Scientific Sonic Dismembrator Model 500 at 15% amplitude
Lynch and Gall, 2006
The molecular kinetic energy is higher, meaning reactions happen faster
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