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Botox is injected into the muscles around the head and neck to reduce muscle tension
However, we can outline the most common and widely accepted protocols used in preclinical studies
To assess binding to a panel of RBD mutants, HIS1K biosensors (FortBio) were equilibrated in assay buffer (PBS) for 15 s before loading of His-tagged SARS-CoV-2 RBD, VoC RBDs, or SARS-CoV-1 RBD (30 g ml 1 diluted in PBS) for 100 s
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