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& Savvides, S

Membranes were blocked for 1 h at room temperature in 3% BSA prepared in TBS-T (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 0.05% Tween 20)

Moreover, artesunate induces the G2/M cell cycle blockade in HFL-I and H460 cells and blocks the cell cycle in the G0/G1 phase in H1975 and LLC cells

However, these patients simultaneously exhibit increased concentrations of proinflammatory lipid mediators, including thromboxane B2, prostaglandins, and LTs, indicative of a lipid mediator storm characterized by an imbalance between proinflammatory and pro-resolving signals

For the amplification of GLUT-4 and Ppia , specific primers were used with the following real-time PCR steps: an initial denaturation step followed by 40 cycles of denaturation, annealing, and extension