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Moreover, smooth-fitting curve analysis identified a threshold effect: below 358.7 pg/mL, each 10 pg/mL increase in vitamin B12 was associated with a 4.4% increase in clinical pregnancy rate (OR = 1.04, P = 0.03), beyond which no further benefit was observed
The absorbance change at 540 nm was plotted for B 12 binding and the absorbance change at 700 nm was plotted for BV binding

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Sikiric et al., 2011

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