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However, these uncontrolled observations require validation through prospective, controlled clinical trials before establishing standard treatment durations for specific clinical indications

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Cell-culture (3T3-L1 adipocytes) 5-Amino-1MQ at 30 M strongly reduced 1-MNA levels in mouse fat cells, confirming NNMT inhibition (Neelakantan et al., Biochemical Pharmacology, 2018)

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Tissue sections, 5 m thick, were cut using a Cryostat microtome (Leica Biosystems, Germany) and mounted onto Silane-coated slides

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