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After a 2D classification with a binned dataset in CRYOSPARC, a homogeneous subset of particles was re-extracted as the native pixel sampling and subjected to Bayesian particle polishing in RELION, followed by another further 2D classification in cryoSPARC

Further complicating its application are challenges related to catalases stability under harsh conditions, limited cellular uptake, and the potential for immunogenic responses, all of which can impact its efficacy and safety profile

Conversely, excessive Cu can generate OH via the Fenton reaction, inducing oxidative stress and cellular damage [97]