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Aging 121 GiulianiD.MioniC.AltavillaD.LeoneS.BazzaniC.MinutoliL.et al (2006)

Although molecular docking revealed that atractylon can bind to sirtuin 3, the detailed mechanism by which atractylon controls the expression of sirtuin 3 remains unclear

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Immunohistochemical detection of p53 and progerin, were performed on paraffin sections (3 m), and subsequent sections were exposed to horseradish peroxidase (HRP)-antibody colored with DAB, and visualized by microscopy (BX51, Olympus, Japan)