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We then verified the correct targeting of the respective sensors by cellular fractionation and mitochondrial subfractionation (Figure 2A)

Analysis of apoptosis in relation to tissue destruction associated with hashimotos autoimmune thyroiditis

N., Belemkar S., Ojha S., et al

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Stained samples were mounted in SlowFade Gold antifade reagent (Thermo Fisher Scientific) and observed under a Zeiss LSM710 or LSM880 confocal laser-scanning microscope

Four solutions were prepared: 100 % methanol, methanol/water (50:50) (v/v), methanol + 0.01 % acetic acid, and ammonium buffer pH 3.5 [H 2 O/ACN (90:10) (v/v) + 1 mM NH 4 (CH 3 COO)]