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A 21G is commonly used but selection depends on the viscosity of the liquid being injected (Dougherty and Lister, 2015)

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For the vast majority of people, the answer is a resounding yes

When the concentrations measured by three-phase HF-LPME are used to calculate the toxicities of ionizable organic compounds to organisms, the EC50 under different pH conditions should be the same, enabling estimation of the toxicity irrespective of the ambient pH