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Using water as solvent, adding 2.2mol/L pyridine and 10mol/L potassium hydroxide (PH = 12-12.5), adding slightly excess phosphorus oxychloride in IAA at 0-10 , and neutralizing with magnesium oxide after refining with cation exchange resin to obtain the product with a yield of more than 70%

The enzyme is encoded by a single, highly conserved gene across species, and its expression is regulated at transcriptional, post-transcriptional, and post-translational levels

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