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Metabololipidomics For oxylipin analysis, 200 L culture supernatants were transferred to 2 mL of ice-cold methanol containing 10 L of deuterium-labeled internal standards (200 nM d8-5S-HETE, d4-LTB 4 , d5-LXA 4 , d5-RvD 2 , d4-PGE 2 and 10 M d8-AA

1O), implying that the lethality of VRK2-deficient PC cells induced by LCS3 scarcely depends on TXNRD1 inhibition

42 Moreover, tissue- and cell-type specific differences in ISC biosynthesis flux and ISC protein stability may also underlie differences in ISC protein levels observed in specific tissues

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