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In Graves disease, about 5% of patients have pretibial myxedema, in which mucopolysaccharide infiltration of the dermis yields marked thickening of the pretibial skin

Bioinformatics analysis 16S-Amplicon processing pipeline The 16S-amplicon processing pipeline was modified from 16S Bacteria/Archaea SOP v1 of Microbiome Helper workflows [31]

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For MS data acquisition, a Q-Exactive Plus mass spectrometer (Thermo Scientific, San Jose, CA, USA) was operated in ESI-positive ion mode, performing a full MS mode from m/z 100-1000

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performed the analysis