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Description
We, next, examined whether inhibition of MEK1/2 leads to suppression of IGF-1R inhibition-induced p70S6K1 activation and promotes cell death

For immunofluorescence, tumor cells grown on poly-lysine-treated coverslides were fixed with 4% paraformaldehyde for 15 min at RT and permeabilized with cold methanol (20 C) for 5 min or with 0.1% Triton X-100/PBS at RT for 15 min before incubated overnight at 4 C with primary antibodies ( Supplementary Table 3 )

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Rotate injection sites to reduce irritation
Glutathione fights free radicals, slowing signs of aging and environmental damage

La Rocque, C
