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Protein lysates were boiled at 95 C for 10 min on a thermomixer (Thermo Fisher Scientific) and sonicated on the Bioruptor (Diagenode) system

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Following this, JC-1 dye (Chem Cruz, SC 364116 A) (1 g/ml in DMSO) was added to them and incubated in the CO 2 incubator for 30 min
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Only 4-7% of participants in SURMOUNT-1 and 2 discontinued the medication due to AE (Tables 2, 3)
