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Cell culture Erythroid progenitors from SCD patients were generated with peripheral blood mononuclear cells (PBMN) followed by CD34+ cell selection (#17856, StemCell Technologies)

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In addition, local bleeding can also be seen during surgical resection, probably due to coagulation deficits caused by heparin release [66]

These add substances like tramadol, fentanyl, or synthetic cannabinoids

For antibody staining, antigen retrieval was performed on the slides by incubating them in a steamer for 40 min in citrate buffer (Vector Labs, H-3300-250)

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